Ms Yunke Huang
China Medical University Shenbei Campus,China
Title: CSS(Chai-hu-shu-gan-san) and therapeutic compounds treating acute-forced-swim-induced brain-vas-gut-ovary axis dysfunction: redefining one-disease depression as multimorbidity model and its holistic strategy
Biography
Biography: Ms Yunke Huang
Abstract
Background: Brain-vas-gut-ovary disorders under stress had been a hidden secret of multimorbidity(as Gan-axis dysfunction described in Traditional Chinese Medicine(TCM)) in single-disease-single-target framework, causing unsatisfactory therapeutic effects.
Method:Female SD rats weighing between 215-230g were grouped into: blank group, model group(AFS(acute forced swim) for 15 min)，CSS(Chai-hu-shu-gan-san) group, MH(meranzin hydrate) group, FA (ferulic acid) group, MH+Dlys(inhibitor of ghrelin) group, n=6. 24h after modeling and intragastric administration, OFT(open field time), IT(immobility time) were tested for depression, and indices to systematically describe brain-vas-gut-ovary dysfuncion(BOLD-fMRI of regulated brain region/velocity time integral for vascular impairment/gut flora 16sRNA sequencing analysis/estrogen level detection for ovary) were examined.
Results: While causing obvious depression with OFT decreased from 14199.80±893.71mm to 8792.75±2098.27mm(p<0.01), IT increased from 80.12±13.84s to 134.49±8.38s(p<0.01)，AFS also synchronously induced blood oxygen levels increase in corpus striatum, cingulate gyrus etc brain region; VTI(velocity time integral) declined from 29.47 ± 6.8mm to 16.42 ± 1.49mm; composition of gut flora in stomach and duodenum changed with lifted Lactobacillus etc. proportion and descended Romboutsia etc. proportion on genus level; E2 dropped from 1279.48pg/ml to 1194.08pg/ml. All these influences were revised by using CSS and their therapeutic compounds, while the curative effects were inhibited by Dlys.
Conclusions: AFS caused brain-vas-gut-ovary(together as Gan axis) multimorbidity instead of depression alone, and these effects could be alleviated by CSS and its therapeutic compounds, during which ghrelin might be the critical cooperative molecule.